Movie 3.

75 min of actin cable growth in a SiR-Actin labeled wild type (w¹¹¹⁸) stage 10B egg chamber treated with 100 μM of the formin inhibitor SMIFH2. Images were taken every 15 min. This video illustrates two significant differences we observed with inhibitor treatment of wild type egg chambers. For the first 30–35 min in the inhibitor, cable growth proceeds as normal (yellow), but by approximately 45 min the cables stop growing (cyan; Fig. 6A-C). In addition, some cables bent as they grew (magenta; Fig. 6G,H). Each frame is a maximum projection of 16 optical slices at 0.5 μm step size.