Movie 4.

120 min of nuclear movement in a SiR-Actin and Hoechst labeled wild type (w¹¹¹⁸) stage 10B egg chamber. Images were taken every 15 min. Each frame is a maximum projection of 5 optical slices at 0.5 μm step size. This is an example of the type of data we used to calculate the nuclear movement trajectories and distances shown in Fig. 8K. In this example, we used ImageJ (Fiji v.1.53q) to calculate the cell centroids (black dots) using the actin images and the nuclear centroids (cyan dots) using the nuclear images for each frame. For the data in Fig. 8K, only the 0 min and 90 min time points were used to calculate trajectory and distance (see Methods for details). Thus, in our quantification, we did not track the specific paths that the nuclei take like we did in this example. More detailed tracking of nuclear paths in future experiments might generate novel insights into how the actin cable arrays guide nuclear movement.