Movie 11

(Main figure 5D): Confocal time-lapse movie showing lumen formation in the ISV and DLAV from 34 hpf onwards in a wild-type embryo. Endothelial cells are labeled by cytoplasmic RFP (Tg(fli1a:gal4ff)^ubs3; (UAS:mRFP)) and endothelial cell junctions in green (VE-cad-Venus) ((Tg(cdh5:cdh5-TFP-TENS-Venus)^uq11bh).