Movie 21

(Supplementary figure 4): Confocal time-lapse movie showing dynamic re-localization of Pecam-EGFP (Tg(fli1a:pecam1-eGFP)^ncv27) during anastomosis in a wild-type and in a rasip1^ubs28 embryo, starting at 32 hpf and recorded at 1 frame/min (00:00 to 00:43). In the rasip1^ubs28 mutant, a defect in the localization of apical junctional proteins was observed. Scale bars, 5 μm.