Movie 1.

Live-cell fluorescent imaging of early cleavage divisions. Bovine zygotes were microinjected with fluorescently labeled modified mRNAs to mCitrine-Actin (green) and mCherry-Histone H2B (red) to distinguish blastomeres and DNA, respectively, and early mitotic divisions visualized by live-cell confocal microscopy. Note the micro-/multi-nuclei in embryos #3, #4, and #11, chromatin bridge in embryo #1, lack of syngamy in embryos #3 and #11, multipolar divisions in embryos #1, #3-6, #11, and #15, and production of empty blastomeres in embryos #5 and #15.