Movie 1

Time-lapse imaging of embryos expressing EGFP-hRAB5A and mGlyT1amCherry was performed to obtain DIC and fluorescent images at 8 hpf. Images were taken at intervals of 20 min to the blastocyst stage. The maximum intensity projections of the confocal micrographs are indicated. Scale bar: 50 μm.

Clathrin-mediated endocytosis is essential for the selective degradation of maternal membrane proteins and preimplantation development

Akihito Morita, Yuhkoh Satouh, Hidetaka Kosako, Hisae Kobayashi, Akira Iwase, and Ken Sato

Development 2021. 148:None-None; doi: 10.1242/dev.199461