Movie 1.

Cellular cortex of a kpna7 maternal crispant and wild-type embryo at 6 hpf. Individual focal planes of confocal Z-stack of a 6 hpf embryo (shown as a 2D Z-projection in Figure 4B). The cell membranes are labeled with anti-β-catenin antibodies (red) and DNA is stained with DAPI (blue). Visualization of sequential focal planes demonstrates that the kpna7 maternal crispants contain cells that lack nuclei.

Identification of maternal-effect genes in zebrafish using maternal crispants

Cara E. Moravec, Gabriella C. Voit, Jarred Otterlee, and Francisco Pelegri

Development 2021. 148:None-None; doi: 10.1242/dev.199536