Movie 2.

FRaeppli-NLS labelling of adult tissues. Endogenous FRaeppli-NLS fluorescence is well preserved with SeeDB2 clearing and detectable beyond 120 μm, while the fluorescent signal disappears after only 30 μm of depth without clearing (n=2).

FRaeppli: a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish

Sara Caviglia, Iris A. Unterweger, Akvilė Gasiūnaitė, Alexandre E. Vanoosthuyse, Francesco Cutrale, Le A. Trinh, Scott E. Fraser, Stephan C. F. Neuhauss, and Elke A. Ober

Development 2022. 149:None-None; doi: 10.1242/dev.199615