Time-lapse imaging of neutrophil recruitment to a scale removal wound in an intubated adult zebrafish. 0–4″ Schematic diagram an adult casper Tg(lyz:DsRed2)NZ50;Tg(mrc1a:eGFP)y251 double transgenic zebrafish with fluorescent neutrophils (magenta) and lymphatic vessels (green). The approximate site of scale removal by abrasion with a scalpel is noted with a yellow box. 5–10″ Adult fish being imaged in the intubation chamber on an inverted confocal microscope, using blue, green and purple (near-UV) excitation light. 11–31″ Time-lapse imaging of the wound site of an adult fish collected with a 2X objective from 0–19 hours post wounding and intubation 31–38″ Close-up of the wound site collected with a 10X objective at 19–20 hours post wounding and intubation. 38–44″ High magnification closeup of neutrophils (magenta) actively migrating in and around lymphatic vessels (green) in the recovering wound site of a live adult zebrafish collected with 20X objective from 23–24 hours post wounding and intubation.
Daniel Castranova, Bakary Samasa, Marina Venero Galanternik, Aniket V. Gore, Allison E. Goldstein, Jong S. Park, and Brant M. Weinstein
Development
2022. 149:None-None; doi: 10.1242/dev.199667