Movie 2.

Repeated time-lapse imaging of neutrophil recruitment to a scale removal wound in an intubated adult zebrafish. 0–14″ Schematic diagram showing experimental setup: an adult casper Tg(lyz:DsRed2)NZ50;Tg(mrc1a:eGFP)y251 double transgenic zebrafish with fluorescent neutrophils (magenta) and lymphatic vessels (green) is wounded, intubated and imaged for 3.5 hours then returned to the aquaculture system. Imaging and intubation (3.5 hours) is repeated at day 1, 2, and 7 post injury. 15–25″ Time lapse, 0.5–4 hours post injury. 25–34″ Time lapse, 24–27 hours post injury. 35–42″ Time lapse, 48–51.5 hours post injury. 43–52″ Tile image of entire intubated fish at 7 days post injury, zooming to wound site. 53-1:09″ 3.5 hour time lapse of wound site at 7 days post fertilization including increased magnification.

Long-term imaging of living adult zebrafish

Daniel Castranova, Bakary Samasa, Marina Venero Galanternik, Aniket V. Gore, Allison E. Goldstein, Jong S. Park, and Brant M. Weinstein

Development 2022. 149:None-None; doi: 10.1242/dev.199667