Movie 3.

Intubation and imaging of Mexican Cavefish (Astyanax mexicanus – Pachon). 0–6″ A six-month old cavefish swimming before intubation. 7–12″ Cavefish intubated and being imaged on an inverted spinning disk confocal microscope. 13–17″ Overview of 405 nm autofluorescence tile zooming in to scale/lateral line region. 18–27″ 3D rendering of scale lateral line region. 28–33″ Pan from scale to preoperculum. 34–41″ Transmitted light movie (black and white) in real-time showing blood flowing through the preoperculum. 42–49″ Pan from preoperculum to maxilla. 50–57″ Transmitted light movie (color) in real-time showing blood flowing through maxilla. 58-1:02 Pan from maxilla to entire mouth showing intubation. 1:03–1:23″ Transmitted light movie (sped up 15X) showing cavefish being revived with fresh system water. 1:24–1:35″ Cavefish swimming after 3.5 hour intubation.