Movie S2.

A single C. elegans larva developing from overnight starvation up to the early L2 stage. Displayed are maximum intensity projected epifluorescence images (total height of projection is 1.5μm), in otherwise unprocessed format. Visible are both seam cell division and fusion as well as P-cell migration toward the ventral midline and division. Features are outlined using the DLG-1::GFP marker at 15-minute intervals for a total of 25 hours.