Movie S5.

Top view: Z-projection of the developing vulva from the L3 stage up to the L4/adult transition. Bottom view: X-projection of the same vulva over time. Both views show the initial division of the VPCs followed by invagination, formation and enlargement of the toroids followed by collapse during eversion. Epifluorescence images displayed after cropping, deconvolution, registration and projection. Features are outlined through the HMR-1::GFP marker (hypodermis and VPCs) and the mCherry::moeABD marker (outlining the AC).