Movie 12.

The anterior centrosome associates with the spectrosome in mitosis. Centrosome separation occurs early in G1. 75-minute time-lapse movie of a GFP::asl, GFP::par-1, his::RFP germarium showing the association of the GSC anterior centrosome with the spectrosome at t = –6′. Subsequently, the centrosome translocates to the GSC cortex adjacent to the cap cells (t = 6′). At t = 36′, the centrosome splits and two GFP::Asl-positive dots are observed. Note that the sibling CB also undergoes centrosome separation soon after the GSC event (t = 43.5′). t = 0′ corresponds to NEP. Yellow arrow: GSC spectrosome; yellow open arrowheads: GSC centrosomes; CB: cystoblast; orange open arrowheads: CB centrosomes. The movie is a maximum projection of 5–9 z-planes (1 μm each) per time point (taken every 1.5 minutes). Related to Fig. 5B.