Movie 13.

GSC centrosomes separate before centriole duplication. 121-minute time-lapse movie of an EB1::GFP, YFP::cnb germarium to label nucleating microtubules and daughter centrioles, respectively. The movie shows a GSC that, upon division, contains only one YFP::Cnb-positive dot, at least until 108′ after NEP. White open arrowheads: GSC centrosomes. The movie is a maximum projection of 5–9 z-planes (1 μm each) per time point (taken every 1.5 minutes). Related to Fig. 6A.

Development ; doi: 10.1242/dev.199716