Movie 14.

Colcemid treatment disrupts microtubule meshwork integrity and affects the pulsatility of the amnioserosa cells. The apical microtubule cytoskeleton (visualized using Jupiter GFP) and constriction pulses of amniserosa cells (visualized using Ecad GFP) upon colcemid treatment. The panels in B are zoomed in views of the images in A. Scale bar -20 μm (A) and 10 μm (B). (Related to Figs 8, S7)

The microtubule end-binding proteins EB1 and Patronin modulate the spatiotemporal dynamics of myosin and pattern pulsed apical constriction

Anwesha Guru, Surat Saravanan, Deepanshu Sharma, and Maithreyi Narasimha

Development 2022. 149:None-None; doi: 10.1242/dev.199759