Movie 5.

Apical microtubule reorganise around apicomedial myosin during pulsed apical constriction. Microtubule minus ends are visualized by Patronin GFP and myosin by Sqh mCherry during a cycle of motile (A) and stationary (C) myosin blob during pulsed apical constriction. Microtubule plus ends are visualized by EB1 GFP and myosin by Sqh mCherry during a cycle of motile (B) and stationary (D) myosin blob during pulsed apical constriction. Red arrows shows reorganisation of EB1 (B, D) or Patronin (A, C). Yellow and white arrowheads mark the movement of myosin blobs. White and red line marks the cell outline in D. Scale bar -5 μm. (Related to Fig. S3, S4)

The microtubule end-binding proteins EB1 and Patronin modulate the spatiotemporal dynamics of myosin and pattern pulsed apical constriction

Anwesha Guru, Surat Saravanan, Deepanshu Sharma, and Maithreyi Narasimha

Development 2022. 149:None-None; doi: 10.1242/dev.199759