Movie 4.

3D fusion reconstruction of four different angles at 40x magnification. Animation of a 3D reconstruction after fusion of images acquired from four angles (0°, 90°, 180° and 270°) by the MuViScope of an Ecad:3xGFP Drosophila pupa at 24 hAPF with a 40x objective to achieve cellular resolution. At each angle, 25 tiles (5x5) were acquired to cover the full dissected part of the animal. Each tile was then deconvolved with the Huygens software and then stitched with the Pairwise Stitching plugin (Preibisch et al., 2009). The animation starts with a dorsal view of the notum and the head, followed by a zoom cellular resolution on the notum and finishes by a 360° rotation around the A-P axis. The acquisition parameters are detailed in Table S1. Scale bar: indicated on the film according to the zoom factor (in μm).