Time-lapse imaging of wt and dpyOV1 pupae. The A-P and L-R axes are indicated on the dorsal view while the A-P and D-V axes are indicated on the lateral view. Wing and notum tissues were imaged in wt and dpyOV1 pupa expressing Ecad:3xGFP. Points of interest are indicated with arrows. Green arrowheads: time-point when distal wing is similarly shaped in both conditions. Yellow arrowheads: position of the anterior comma like invaginations in Ecad:3xGFP, dpyOV1. The arrowhead points to approximately the same position in Ecad:3xGFP, wt for comparison. Red arrowheads: position of a lateral pit in Ecad:3xGFP, dpyOV1. The arrowhead points to approximately the same position in Ecad:3xGFP, wt for comparison. The notum and wing were imaged respectively with 10x and 40x objectives at 0° and 90° with one position for 10x and two not-tiled distinct positions at 40x.The acquisition parameters are detailed in Table S1. Scale bars 50 μm at 40x and 100 μm at 10x.
Olivier Leroy, Eric van Leen, Philippe Girard, Aurélien Villedieu, Christian Hubert, Floris Bosveld, Yohanns Bellaïche, and Olivier Renaud
Development
2022. 149:None-None; doi: 10.1242/dev.199760