Movie 8.

Time-lapse imaging of wt and dpyOV1 pupae. The A-P and L-R axes are indicated on the dorsal view while the A-P and D-V axes are indicated on the lateral view. Wing and notum tissues were imaged in wt and dpyOV1 pupa expressing Ecad:3xGFP. Points of interest are indicated with arrows. Green arrowheads: time-point when distal wing is similarly shaped in both conditions. Yellow arrowheads: position of the anterior comma like invaginations in Ecad:3xGFP, dpyOV1. The arrowhead points to approximately the same position in Ecad:3xGFP, wt for comparison. Red arrowheads: position of a lateral pit in Ecad:3xGFP, dpyOV1. The arrowhead points to approximately the same position in Ecad:3xGFP, wt for comparison. The notum and wing were imaged respectively with 10x and 40x objectives at 0° and 90° with one position for 10x and two not-tiled distinct positions at 40x.The acquisition parameters are detailed in Table S1. Scale bars 50 μm at 40x and 100 μm at 10x.

Multi-view confocal microscopy enables multiple organ and whole organism live-imaging

Olivier Leroy, Eric van Leen, Philippe Girard, Aurélien Villedieu, Christian Hubert, Floris Bosveld, Yohanns Bellaïche, and Olivier Renaud

Development 2022. 149:None-None; doi: 10.1242/dev.199760