Movie 9.

Comparison of average tissue flow during pupal development upon acquisition by MuViScopy or spinning disk microscopy. Average tissue flow between 17 and 26.45 hAPF in the dorsal thorax epithelium of Drosophila pupae Ecad:3xGFP imaged by MuViScopy (n=3) or spinning disk microscopy (n=3) using a 40x objective. The averaged movies were generated upon time-lapse movie registration and rescaled using the macrochaetae (yellow dots). The red cross indicates the barycenter between the macrochaetae. The midline is indicated by a grey dashed line. Tissue flow is quantified by velocity vectors measured by PIV (Guirao et al., 2015) and averaged over 2 h. The scale bar represents a displacement of 5 μm/h.