Automatic tracking of cells in the early Drosophila embryo in which nuclei are labelled with NaNuTrap method. Cells in Movie 2 (i.e., Kr-Gal4 driving expression of NNT in presence of EGFP deposited maternally) tracked automatically without manual correction using the Imaris software tracking function. Spot detection and tracking can be initiated earlier compared to conventional cell labelling methods as cell nuclei are labelled more effectively using NNT (e.g. see Movie 2). Color bar indicates average speed of the tracks (0‘4.5 μm/min).