Dynamics of VNP expression sorted Gata6VNP-positive cells cultured in N2B27 supplemented with PD03. Time-lapse imaging of Gata6VNP reporter cells flow sorted for VNP expression 16 h after the end of a doxycycline pulse and cultured in defined N2B27 medium supplemented with 1 ìM of the MEK inhibitor PD03. Frame rate is 10 min.