Movie 5.

A representative time-lapse recording of a SN-EGFP-labeled epiblast from st. 4 to st. 9 with a graft of mCherry-labeled st. 4 Japanese quail node at an anterolateral position of the host node. This movie shows the node graft development into an ectopic graft-derived AME (gAME) and the convergence of the surrounding epiblast cells toward the gAME, which elicited the secondary brain tissue development, followed by its fusion to the host brain. The frames were at 25 min intervals and shown at 6 fps. The original data for Figure 5(A)(a') to (d').

Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development

Koya Yoshihi, Kagayaki Kato, Hideaki Iida, Machiko Teramoto, Akihito Kawamura, Yusaku Watanabe, Mitsuo Nunome, Mikiharu Nakano, Yoichi Matsuda, Yuki Sato, Hidenobu Mizuno, Takuji Iwasato, Yasuo Ishii, and Hisato Kondoh

Development 2022. 149:None-None; doi: 10.1242/dev.199999