Movie 7.

A representative time-lapse recording of a SN-EGFP-labeled epiblast from st. 4 to st. 9 with a graft of mCherry-labeled st. 5 Japanese quail AME at an anterolateral position of the host node. The AME graft developed further into the anteriormost prechordal plate (PP) and more posterior, anterior notochord (ANC). The surrounding epiblast cells converged toward the gAME tissues similarly to following a node graft, formed the secondary brain tissue, and eventually fused to the host brain. The frames were at 10 min intervals and shown at 6 fps. The original fluorescence data for Figure 5(C).

Live imaging of avian epiblast and anterior mesendoderm grafting reveals the complexity of cell dynamics during early brain development

Koya Yoshihi, Kagayaki Kato, Hideaki Iida, Machiko Teramoto, Akihito Kawamura, Yusaku Watanabe, Mitsuo Nunome, Mikiharu Nakano, Yoichi Matsuda, Yuki Sato, Hidenobu Mizuno, Takuji Iwasato, Yasuo Ishii, and Hisato Kondoh

Development 2022. 149:None-None; doi: 10.1242/dev.199999