Movie 8.

The second example of ectopic mCherry-labeled AME graft in SN-EGFP-labeled epiblast field analogous to Movie 7, but the node graft position more remote and lateral to the host node. The secondary brain tissue that developed surrounding the gAME was fused to the host brain only at its posterior end due to head ectoderm development separating the two brains at the anterior positions. Note also the contribution of area opaca-derived cells in the secondary brain. The original fluorescence data for Figure 5(D).