Movie 15.

Central canal cilia at 52 h.p.f. in cfap298tm304 mutants and controls raised at 30°C until 28 h.p.f. then downshifted to 22°C. Images were acquired with a Nikon Ti2 inverted microscope and pco.edge sCMOS camera at 250 frames per second for 4 seconds. The movie is replayed at 50 frames per second. Upper panels show DIC with a small Gaussian blur. Lower panels show background subtracted using a moving average of 55 frames. Scale bar — 5 μm. Left - rostral; top - dorsal.

Development ; doi: 10.1242/dev.200017