Movie 2.

Imaging of R26-H2BmCherry^+/- embryos prior to RNA in situ hybridization chain reaction (HCR) Related to Fig. 1E(ii). An embryo expressing the nuclear marker H2BmCherry (red) was light-sheet imaged from E7.5 for 24 hrs, with Z-stacks with 7.5 μm spacing, 120 slices per sample, 200 ms exposure time with 1.2% 20mW 561nm laser and 10 min imaging interval. The movie shows the embryos as MIP from posterior (top) and distal orientation (bottom). Time is indicated in hr:min.