Movie 5.

Visualizing the pulse and the very first LuVeLu waves. Related to Fig. 2F. The movie shows LuVeLu fluorescence in an embryo imaged from LS stage onwards from posterior side. The embryo is shown as MIP from three perspectives: posterior (top left), left side (top right), distal (bottom left). The distortion of the embryo in the first few frames is an artefact of re-orientating the image stack to align the embryo to the image axes (see Materials and Methods). Note the first, very faint oscillation occurring at 10:00 h. Time is indicated in hr:min.