Movie 3.

Time-lapse imaging of transient interactions between STC receiver and CmGP1GH1 sender cells. Time-lapse imaging of STC clone A1 receiver cells cultured with CmGP1GH1 sender cells at a 1:1 ratio for 24 hours. Brightfield (grey), EGFP (green) and mCherry (red) images taken every 10 minutes over a 24 hour period. Immunofluorescence analysis of tagBFP (blue), EGFP (green) and mCherry (red) expression at the 24 hour timepoint is shown at the end of the movie. All images were acquired using a Nikon Ti-E microscope. Movies 1 and 3 display different fields of view from the same experiment.