Movie 4.

Kinetics of mCherry downregulation in STC receiver cells (0-24h). Timelapse imaging of STC clone A1 receiver cells cultured with CmGP1GH1 sender cells at a 1:9 ratio for 24 hours, then treated with 1μg/ml doxycycline (to inhibit mCherry transcription) and imaged for 24 hours. Brightfield (grey), EGFP (green) and mCherry (red) images taken every 10 minutes over a 24 hour period, using a Nikon Ti-E microscope.