Movie 5.

Kinetics of mCherry downregulation in STC receiver cells (24-48h). Timelapse imaging of STC clone A1 receiver cells cultured with CmGP1GH1 sender cells at a 1:9 ratio for 24 hours, then treated with 1μg/ml doxycycline (to inhibit mCherry transcription) and imaged between 24 and 48 hours following addition of doxycyline. Brightfield (grey), EGFP (green) and mCherry (red) images taken every 10 minutes over a 24 hour period, using a Nikon Ti-E microscope.

Development ; doi: 10.1242/dev.200226