Movie 4.

Spontaneous Ca2+ activity in the midbrains of sibling and Mutcq71/cq71 mutant embryos. Related to Figure 1. Simultaneous calcium imaging of neuronal cells in 3dpf midbrains of Tg(HuC:GCaMP6S) embryos. Acquisitions were performed with scanning at 1.2 Hz at an excitation of 488 nm. The resolution was designed by 512 × 512 pixels on Tg(HuC:GCaMP6s). And the time-length of single-layer imaging was 252 seconds in total, with 200 cycles. The selected fame images were given in Figure 1C.

Development ; doi: 10.1242/dev.200342