Movie 3.

Apical delamination of a wt1b-overexpressing cardiomyocyte in a cardiac ventricle at 5 dpf. In vivo time-lapse imaging of a myl7:Gal4;eGFP:UAS:wt1b heart between 5 and 6 days post fertilization (dpf) acquired with the Leica TCS SP8 DLS confocal microscope, using the digital light sheet (DLS) mode. Shown is the reconstruction of a single plane of the beating ventricle. Note how the extruded cells flatten down during the time course of the Movie (Yellow arrow). Scale bar, 50 μm.

Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium

Ines J. Marques, Alexander Ernst, Prateek Arora, Andrej Vianin, Tanja Hetke, Andrés Sanz-Morejón, Uta Naumann, Adolfo Odriozola, Xavier Langa, Laura Andrés-Delgado, Benoît Zuber, Carlos Torroja, Marco Osterwalder, Filipa C. Simões, Christoph Englert, and Nadia Mercader

Development 2022. 149:None-None; doi: 10.1242/dev.200375