Movie 3.

Apical delamination of a wt1b-overexpressing cardiomyocyte in a cardiac ventricle at 5 dpf. In vivo time-lapse imaging of a myl7:Gal4;eGFP:UAS:wt1b heart between 5 and 6 days post fertilization (dpf) acquired with the Leica TCS SP8 DLS confocal microscope, using the digital light sheet (DLS) mode. Shown is the reconstruction of a single plane of the beating ventricle. Note how the extruded cells flatten down during the time course of the Movie (Yellow arrow). Scale bar, 50 μm.