Movie 6.

Serial block face scanning z-stacks through a wt1b-overexpressing heart at 5 dpf. Serial stacks through a ventricle from a myl7:Gal4;eGFP:UAS:wt1b embryo at 5 dpf. Images were obtained by serial block face scanning electron microscopy. Note the absence of a compact and organized myocardial layer and the enlarged cardiac jelly separating the endocardium and the myocardium. Also visible the extensive areas filled with extracellular matrix. EpC, epicardial cell, EnC, endothelial cell, Ery, erythrocyte, CM, cardiomyocyte, ECM, extracellular matrix, v, ventricle, at, atrium. Scale bar, 20 μm.

Wt1 transcription factor impairs cardiomyocyte specification and drives a phenotypic switch from myocardium to epicardium

Ines J. Marques, Alexander Ernst, Prateek Arora, Andrej Vianin, Tanja Hetke, Andrés Sanz-Morejón, Uta Naumann, Adolfo Odriozola, Xavier Langa, Laura Andrés-Delgado, Benoît Zuber, Carlos Torroja, Marco Osterwalder, Filipa C. Simões, Christoph Englert, and Nadia Mercader

Development 2022. 149:None-None; doi: 10.1242/dev.200375