Movie 1.

Apical surfaces of neuroepithelial structures in a 2-month-old brain organoid. The movie shows the 3D data of the organoid shown in Fig. 4. Staining was against cell nuclei (Hoechst, cyan) and ZO1 (magenta). The last phase of the movie shows the cropped apical surfaces of the neuroepithelium inside the organoid. The color labeling was randomly generated according to surface area.

Imaging three-dimensional brain organoid architecture from meso- to nanoscale across development

Juan Eduardo Rodriguez-Gatica, Vira Iefremova, Liubov Sokhranyaeva, Si Wah Christina Au Yeung, Yannik Breitkreuz, Oliver Brüstle, Martin Karl Schwarz, and Ulrich Kubitscheck

Development 2022. 149:None-None; doi: 10.1242/dev.200439