Movie 3.

Pre- and postsynaptic structures in a 14-month-old brain organoid. The movie shows the 3D data and renderings of Fig. 8. The organoid contained 10% EGFPexpressing cells and was labeled by Hoechst and stained with antibodies to the pre- and post-synaptic proteins synapsin 1 (SYN1, green) and Homer1 (red). Please note that in this movie EGFP is shown in blue.

Imaging three-dimensional brain organoid architecture from meso- to nanoscale across development

Juan Eduardo Rodriguez-Gatica, Vira Iefremova, Liubov Sokhranyaeva, Si Wah Christina Au Yeung, Yannik Breitkreuz, Oliver Brüstle, Martin Karl Schwarz, and Ulrich Kubitscheck

Development 2022. 149:None-None; doi: 10.1242/dev.200439