Movie 1.

Time lapse movie showing endothelial Vinculin dynamics in vivo Top: Dorsal aorta (DA) of a wild-type embryo, expressing Tg(fli1ep:Vinculinb-eGFP)^uq2al, showing dynamic Vinculin expression Focal Adhesions and cell-cell junctions over the course of the movie, from 50 hpf to 51 hpf. Bottom: DA of a tln1uq1al^-/- mutants, expressing Tg(fli1ep:Vinculinb-eGFP)^uq2al, showing a dramatic reduction of Focal Adhesions and diffuse cell-cell junctions expression, from 50 hpf to 51 hpf. Scale bar = 10μm.