Movie 3.

Time-lapse imaging during ISVs remodelling Time-lapse imaging of ISVs in a wild-type embryo (left) and a tln1uq1al^-/- mutant (right), showing disconnection of the ISV in the mutant. The endothelial F-actin marker line, Tg(fli1ep:lifeact-eGFP)^uq3al, was used to visualise the vasculature. Arrow indicates ECs disconnecting in the ISV of tln1uq1al^-/- mutant. Movie taken from 30 hpf to 48 hpf. Scale bar = 25μm.