Movie 2.

Depletion of Dlg1 delays the transition from apical constriction to invagination (cross-section view). A dlg1 RNAi embryo (right) shows delayed transition from apical constriction to invagination. A wildtype embryo is shown on the left for comparison. The cross-section views were generated by re-slicing the 3D image stacks acquired over time. Cell membranes are labeled with E-cadherin-GFP. T = 00:00 (mm:ss) marks the onset of gastrulation.