Movie 3.
The apical myosin network behaves normally in the constricting cells of dlg1 RNAi embryos. Confocal images of a representative control and dlg1 RNAi embryo expressing Sqh-mCherry (magenta) and E-cadherin-GFP (green). Maximum projections of the en face view for SqhmCherry and a single apical plane for E-cadherin-GFP are shown. Apical myosin accumulation and the spatial organization of the myosin network are comparable between the control and the dlg1 RNAi embryos during apical constriction. T = 0:00 (mm:ss) marks the onset of gastrulation.
The cell polarity determinant Dlg1 facilitates epithelial invagination by promoting tissue-scale mechanical coordination
Development 2022. 149:None-None; doi: 10.1242/dev.200468