Movie 5.
Laser ablation of the flanking, non-constricting cells delays the transition from apical constriction to invagination. Laser ablation of two strips of flanking cells adjacent to the constriction domain in wildtype embryos during apical constriction. A stage matched, non-ablated embryo is shown on the left for comparison. Top: cross-section view; Bottom: ventral surface view. Cell membranes are visualized using E-cadherin-GFP. T = 00:00 (mm:ss) corresponds to the transition from apical constriction to invagination in control embryos.
The cell polarity determinant Dlg1 facilitates epithelial invagination by promoting tissue-scale mechanical coordination
Development 2022. 149:None-None; doi: 10.1242/dev.200468