Movie 1, related to Figure 1.
Leading centripetally migrating cells appear to reduce contact with the basement membrane. A series of two time-lapse Movies consisting of the data from Fig. 1F,G showing that leading centripetally migrating cells appeared to reduce contact with the basement membrane as migration proceeded. Part 1 shows a stage 10B egg chamber over the course of 2.7 hours with a membranelocalized fluorophore (Myr::tdTomato) and Vkg-GFP. Part 2 shows a stage 10B egg chamber over the course of 3.1 hours with a photoconvertible membrane-localized fluorophore (Myr::tdEOS) that has been photoconverted in the leading centripetal cells. Yellow arrows indicate leading FCs as they reduce contact with the basement membrane. Full genotypes for this Movie are available in Supplemental Table 2. Scale bars 25μm. Anterior is oriented to the left, and dorsal is up.