Movie 3, related to Figure 5.

Knockdown of shotgun in follicle cells resulted in abnormal centripetal migration. A series of six time-lapse Movies consisting of the data from Fig. 5A-F showing phenotypes resulting from clonal knockdown of shg (parts 1 through 4) versus luciferase controls (part 5 and 6). Cross-sectional views are shown, with anterior oriented to the left and dorsal oriented up, except for part 6. Clones are created with the Flipout-Gal4 system and co-express UAS-regulated RNAi transgenes and GFP (magenta). Yellow arrows denote centripetal FCs of interest as indicated in Fig. 5. Phenotypes observed are as described in Fig. 5. Full genotypes for this Movie are available in Supplemental Table 2. Scale bars 25μm.

Two phases for centripetal migration of Drosophila melanogaster follicle cells: initial ingression followed by epithelial migration

Travis T. Parsons, Sheila Mosallaei, and Laurel A. Raftery

Development 2023. 150:None-None; doi: 10.1242/dev.200492