Movie 4, related to Figure 7, S8.

Reduction of basal surface area is visible at the surface of stage 10B egg chambers undergoing centripetal migration. A series of two time-lapse Movies consisting of the data from Fig. S7A-F of stage 10B egg chambers undergoing centripetal migration as viewed from the surface. Egg chambers are labeled with Sqh-mCherry (white) and clonally express luciferase (part 1) or shg UAS-regulated RNAi transgenes (part 2) via the Flipout-Gal4 system (magenta). In video #1, the anterior-most centripetal FCs expressing luciferase UAS-regulated RNAi transgenes reduced their basal surface area at the surface of the egg chamber at a rate comparable to neighboring cells, whereas in video #2 columnar FCs expressing shg UAS-regulated RNAi transgenes appeared to be delayed in this process. Full genotypes for this Movie are available in Table S2. Scale bars 10μm.

Two phases for centripetal migration of Drosophila melanogaster follicle cells: initial ingression followed by epithelial migration

Travis T. Parsons, Sheila Mosallaei, and Laurel A. Raftery

Development 2023. 150:None-None; doi: 10.1242/dev.200492