Movie 1.

ISLV growth in a wild type embryo. Confocal time-lapse imaging from 60 to 70 hpf of the trunk of a lyve1b:DsRed; kdrl:EGFP embryo displaying lymphatics (white) and blood vessels (green). Yellow arrowhead indicates the ISLV tip. Time-lapse image z-stacks were collected 15 minutes apart; movie was made at 6 frames per second. Stills from movie displayed in Fig. 2. Scale bar = 50 μm.