Movie 2.

ISLV growth in a plxnd1 embryo. Confocal time-lapse imaging from 60 to 74.5 hpf of the trunk of a plxnd1^nz75; lyve1b:DsRed; kdrl:EGFP embryo displaying lymphatics (white) and blood vessels (green). Yellow arrowheads indicates the ISLV tips. Time-lapse image z-stacks were collected 15 minutes apart; movie was made at 6 frames per second. Stills from movie displayed in Fig. 2. Scale bar = 50 μm.