Movie 1.

Depth-scroll through individual Z-slices from a confocal Z-stack of labeled wnt8a RNA particles and microtubules in a wild-type embryo. Labeling of wnt8a RNAcontaining particles and microtubules at the vegetal cortex of a 20 mpf wild-type embryo, showing wnt8a RNA localizing to microtubules. This is the same confocal Z-stack displayed as a maximum projection in Fig. 6H; scrolling through multiple Z-slices enables easier observation of the spatial relationship between individual wnt8a RNA particles and microtubules at different embryo depths throughout the entire image.