Movie 1.

Live imaging of NH -Chi and +Chi gastruloids between 102-115h of differentiation. WNT pathway inhibitors XAV939 and LGK974 were used to test the dependence of elongation on WNT pathway activity. Fluorescence signal shows T::H2B-mCherry reporter activity.

Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids

Natalia López-Anguita, Seher Ipek Gassaloglu, Maximilian Stötzel, Adriano Bolondi, Deniz Conkar, Marina Typou, René Buschow, Jesse V. Veenvliet, and Aydan Bulut-Karslioglu

Development 2022. 149:None-None; doi: 10.1242/dev.200679