Movie 3.

Ectopically expressed Cxcl12b is sufficient to determine the directionality of ingrown lymphangiogenesis in the cxcl12b mutant. The Tg(kdrl:DenNTR; kdrl:mCherry; fli1:GFP) triple transgenic line was used for the time-lapse image. Note that the blue fluorescent (GFP artificial fluorescence) iLVs ingrow towards the ectopically expressed Venus-p2A-Cxcl12b (right side, green fluorescence, asterisks), but not the Venus control (left side, green fluorescence, asterisks). Durations of left and right imaging were 410 and 1000 minutes, respectively. Dorsal view. Scale bar, 20 μm.