Movie 11.

Neighboring adult fat body cells are often fused to generate multinucleated cells. Representative movie of cell fusion events at the tergite during 71-79 h APF. CD4::tdGFP in adult fat body cells was driven by 31F09-Gal4 plus Ay-Gal4. Arrows indicate cell-cell boundaries that diminished in the subsequent frames. A large migrating cell is likely to be a hemocyte. By 70 h APF, the fly started a muscle contraction, resulting in significant shifts in the x-y coordinates over time. The shifts in time-series images were automatically corrected by the Linear Stack Alignment with SIFT function in the Fiji software (Lowe, 2004). Anterior is to the left, and dorsal is up.